An automated immunoradiometric assay for ferritin.
نویسندگان
چکیده
منابع مشابه
An automated immunoradiometric assay for human thyrotropin.
In this two-site immunoradiometric assay for thyrotropin, developed for use in the "Kemtek 3000" automated radioimmunoassay system, commercially available monoclonal antibody to thyrotropin is labeled with 125I, and the solid-phase antibody is an IgG fraction of sheep antiserum to thyrotropin, covalently coupled to reprecipitated aminocellulose. There are two incubations, totalling 3 h, the sen...
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Rivanol, the cationic salt of an acridine base, has been used as a novel separation procedure in an immunoradiometric assay for human liver ferritin. The separation step is based on the differences in charge and molecular weight between the labelled antibody-ferritin complex and free labelled immunoglobulins. The resultant assay is simple, reproducible and sufficiently sensitive to determine se...
متن کاملPurification and iodination of antibody for use in an immunoradiometric assay for serum ferritin.
Antibodies were purified by affinity chromatography and radioiodinated by a conjugation method for use inthe immunoradiometric assay for serum ferritin. These procedures are simpler and more reproducible than those described earlier, and the radiolabeled preparations were usable for at least 13 weeks.
متن کاملPreparation of prostate specific antigen standards for immunoradiometric assay
Background: Immunoradiometric assay is one of the most common and precise methods for determination of prostate specific antigen (PSA) in clinical laboratories. Usual use of human serum in routine assays has many disadvantages such as easy contamination and precipitation, instability and unavailability. Thus in order to avoid these problems the artificial matrix was used which acts sim...
متن کاملComparison of three procedures for isolating human ferritin, for use as a standard in an immunoradiometric assay.
We evaluated three methods for isolating ferritin for use as a standard, with respect to purity of the products, ease of preparation, and yield. Examination of the respective products by gel filitration on Sephadex G-200 and Sepharose 6B suggested that the preparations isolated by ammonium sulfate and cadmium sulfate precipitation (Method 1) and by ultracentrifugation (Method 2) were homogeneou...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1975
ISSN: 0021-9746
DOI: 10.1136/jcp.28.7.540